Whole-brain connections of glutamatergic neurons in the mouse lateral habenula in both sexes

Background The lateral habenula (LHb) is an epithalamus nucleus that is evolutionarily conserved and involved in various physiological functions, such as encoding value signals, integrating emotional information, and regulating related behaviors. The cells in the LHb are predominantly glutamatergic and have heterogeneous functions in response to different stimuli. The circuitry connections of the LHb glutamatergic neurons play a crucial role in integrating a wide range of events. However, the circuitry connections of LHb glutamatergic neurons in both sexes have not been thoroughly investigated. Methods In this study, we injected Cre-dependent retrograde trace virus and anterograde synaptophysin-labeling virus into the LHb of adult male and female Vglut2-ires-Cre mice, respectively. We then quantitatively analyzed the input and output of the LHb glutamatergic connections in both the ipsilateral and contralateral whole brain. Results Our findings showed that the inputs to LHbvGlut2 neurons come from more than 30 brain subregions, including the cortex, striatum, pallidum, thalamus, hypothalamus, midbrain, pons, medulla, and cerebellum with no significant differences between males and females. The outputs of LHbvGlut2 neurons targeted eight large brain regions, primarily focusing on the midbrain and pons nuclei, with distinct features in presynaptic bouton across different brain subregions. While correlation and cluster analysis revealed differences in input and collateral projection features, the input-output connection pattern of LHbvGlut2 neurons in both sexes was highly similar. Conclusions This study provides a systematic and comprehensive analysis of the input and output connections of LHbvGlut2 neurons in male and female mice, shedding light on the anatomical architecture of these specific cell types in the mouse LHb. This structural understanding can help guide further investigations into the complex functions of the LHb. Supplementary Information The online version contains supplementary material available at 10.1186/s13293-024-00611-5.

There is evidence to suggest that LHb circuitry, specific to cell type and/or projection, plays a crucial role in mediating stress-related disorders [21], parental behavior [22], and social communication [23] in a sex-dependent manner.Additionally, cellular and synaptic mechanisms that promote the activity of LHb glutamatergic neurons have been linked to depressive-like behaviors in animal models [24,25], despite well-documented sex differences in major depressive disorder in clinical reports [26].It remains to be explored whether there are sex differences in the neural circuits of the LHb vGlut2 neurons.Understanding how neural circuits are organized in both sexes is instrumental in comprehending how information is processed in a sex-dependent manner by the LHb.This knowledge could shed light on the potential of LHb as a novel target for therapeutic intervention of affective disorders.
Several studies have utilized anterograde and/or retrograde tracers to investigate the connections of the LHb, either with or without distinguishing between subpopulations of LHb neurons [8,16,[27][28][29].Nonetheless, to date, there is no complete anatomical map of LHb glutamatergic neuron connectivity in mice or any other species.Currently, a comprehensive circuitry connecting the depiction of the LHb vGlut2 neurons on a whole-brain scale in both sexes is still lacking.
To address this gap, we utilized cell-type-specific monosynaptic rabies virus tracings to comprehensively identify the whole-brain connectivity map of LHb vGlut2 neurons.We also used synaptophysin-expressing adenoassociated viruses to label glutamatergic efferent axons, focusing on presynaptic boutons.Furthermore, we compared the rostro-caudal axis of the connectivity structure of the LHb vGlut2 neurons to establish a connectivitybased compartmentalization that may facilitate future functional studies.We also examined and quantified the connectivity of LHb vGlut2 neurons in both sexes to increase our understanding of normal brain structure.In summary, we identified input and output subregions of LHb vGlut2 neurons and acquired whole-brain quantitative results with specific spatial distribution.We classified the output brain subregions according to the presynaptic boutons and analyzed the input-output connective pattern, which showed distinct clusters but roughly similar connective patterns in both sexes.

Animals
All experimental procedures followed ethical guidelines and have been approved by the Institutional Animal Care and Use Committee at Shenzhen Institute of Advanced Technology (IACUC number: SIAT-IACUC-20,221,209-ZKYSZXJJSYJY-RZC-WF-A2072-02).Adult male and female Vglut2-ires-Cre mice (8-12 weeks old; Jackson lab, Strain#: 016963) used in this study are heterozygous with Cre recombinase under the control of the vGlut2 gene.The C57BL/6J mice (8-12 weeks old) in the control experiment were purchased from Zhejiang Vital River Laboratory Animal Technology Co., Ltd.(Hangzhou, China).The mice were group-housed at a controlled temperature of 25 degrees Celsius and had free access to food and water.They were also maintained on a 12:12 h light: dark cycle, with lights on from 8:00 a.m. to 8:00 p.m.

Stereotaxic virus injection
Mice were anesthetized with sodium pentobarbital (100 mg/kg, i.p. injection), and then placed on a stereotaxic apparatus (RWD Co., Ltd., Shenzhen, China).Input tracing experiments were performed on the Vglut2-ires-Cre mice, and C57BL/6J mice were used for the control experiment.Monosynaptic retrograde tracing was achieved by unilaterally injecting a 1:1 mixture of AAV2/9-hSyn-DIO-His-EGFP-2a-TVA-WPRE-pA (viral titer 2.93 × 10 12 vg/ml; BrainVTA Co., Ltd., Wuhan, China, Cat#PT-0210) and AAV2/9-hSyn-DIO-RVG-WPRE-pA (viral titer 2.98 × 10 12 vg/ml; BrainVTA Co., Ltd., Wuhan, China, Cat#PT-0204) into the LHb (AP: -1.75 mm; ML: 0.54 mm; DV: -2.75 mm), at a total volume of 15 nl and a flow rate of 30 nl/min.After injection, the microsyringe was kept in place for 10 min before being withdrawn to avoid leakage, and then the skin was sutured.The animals were allowed to recover for 3 weeks after AAV injection.Then, 30 nl of RV-ENVA-△G-dsRed (viral titer 2.00 × 10 8 infections units/mL; BrainVTA Co., Ltd., Wuhan, China, Cat#R01002) was injected in the same site and expressed for 7 days before the animals were euthanized.To verify the specificity of the viral strategy, we conducted control experiments by injecting a 1:1 mixture of AAV virus encoding TVA and RVG into the LHb in the Vglut2-ires-Cre mice, without RV or followed by injection of RV into the hippocampus 3 weeks later.Alternatively, both TVA/RVG mixture and RV were injected into the LHb in C57BL/6J mice.For mapping inputs of adjacent nucleus, a 1:1 mixture of AAV virus encoding TVA and RVG was injected in the medial habenula (MHb) (AP: -1.75 mm; ML: 0.20 mm; DV: -2.50 mm) or hippocampus (AP: -1.75 mm; ML: 0.55 mm; DV: -2.00 mm) of Vglut2-ires-Cre mice, followed by an injection of RV in the same site 3 weeks later.

Histology and immunostaining
Mice were anesthetized with sodium pentobarbital chloral hydrate (1%, 100 mg/kg, i.p. injection) and then perfused with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA).The brains were subsequently post-fixed overnight at 4 degrees Celsius in 4% PFA and then cryoprotected in 30% sucrose for 2 days.The brains were sliced into 40 μm coronal sections using a cryostat (Leica, Cat#CM1950), with every third section being used for whole-brain input and output imaging.Cells containing rabies virus were counterstained with DAPI (Sigma-Aldrich, Cat#D9542, 1:5000).For the anterograde tracing experiment, tdTomato and GFP signals located in the presynaptic boutons were immunostained to amplify fluorescent signals of boutons.

Imaging acquisition
For whole-brain input and output imaging, the coronal sections were digitally scanned using an automated slide scanner (Olympus, VS120) with a 10 / 0.45 NA objective.For the starter cells imaging, brain slices covering the injection site were acquired through the confocal laser scanning microscope (Olympus, FV3000) with a 40 / 0.95 NA objective.For whole-brain presynaptic boutons imaging and quantification, the same confocal laser scanner was employed to image the innervation of LHb boutons in downstream subregions.Brain slices containing downstream subregions were scanned in a single optical z-section with mosaic stitching covering the structure, and were imaged at a resolution of 0.311 μm/pixel.Three reporter fluorescent proteins (DAPI, EGFP, and tdTomato) were excited by 405 nm, 488 nm, and 594 nm lasers, respectively.

Counting and data analysis
To delineate the boundaries of each brain subregion, all brain slice images were aligned with the Franklin and Paxinos Atlas (fourth edition) and the Allen Brain Reference Atlas.The quantification of the inputs involved manual counting of starter cells, with only cells displaying distinguishable soma being included in the analysis.The number of input neurons in each brain subregion was summarized by layer, normalized by the total number of input neurons in each sample to determine the input proportion, and then compiled to generate quantified whole-brain inputs.
For measuring the output density, the quantification of synaptophysin-EGFP puncta was semi-automated using ImageJ (NIH, Bethesda, MD, USA).The projection signals were detected by setting the threshold for each section to subtract autofluorescence and segment the binary image in ImageJ.The number of positive pixels within each demarcated area was counted, and the output density percent of brain subregions was calculated by dividing the sum of detected pixels in each section by the total signals across the whole brain, excluding the injection site in each mouse.Bouton characteristics were determined by measuring relative bouton size, and calculating the puncta number and occupied area with ImageJ.
Hierarchical clusters are generated using R and RStudio software (Posit., Boston, MA) to further analyze the output pattern.For classifying presynaptic bouton size, number, and occupied area in each brain subregion, clusters were generated using the "factoextra" package with the "kmeans" method.A hierarchical cluster and heatmap were generated to classify the presynaptic innervating feature of whole-brain subregions based on bouton features including size, number, and occupied area.These features were normalized by the "scale" function.Subsequently, the distance was calculated by the "dist" function, followed by clustering with the "hclust" function, and then a heatmap was generated.These mentioned above classified the innervating characteristic of each brain subregion from the LHb, regardless of sexes.
A Sankey plot was utilized to generate a summary diagram of the input and output connective map using the "networkD3" package.Correlation analysis and clustering of input or output patterns in both sexes were conducted specific steps outlined in a previous reference [30].A correlation matrix was calculated using the "spearman" method, hierarchical clusters were generated and a heatmap was generated in RStudio to visualize the results.

Statistical analysis
The data for input proportion and output density were presented as mean ± s.e.m.Differences between groups were analyzed using multiple t-test followed by the Holm-Sidak method for statistical significance.Statistical analyses were conducted using GraphPad Prism 8, and significance levels are indicated as * P < 0.05.

Whole brain mapping of the LHb vGlut2 input in male and female mice
To identify brain-wide inputs to LHb vGlut2 neurons, we employed a modified rabies virus-mediated retrograde tracing strategy in the Vglut2-ires-Cre mutant mice (Fig. 1a).The neurons co-labeled by EGFP and dsRed in the injection site were starter cells (Fig. 1b), while dsRedlabeled neurons without EGFP signals were input cells.Most of the starter cells were within the injection site, predominantly located in the central to posterior part of LHb (Fig. 1c; Additional file 1: Fig. S1; Additional file 2: Fig. S2).Cases were only adopted if the starter cells located in the LHb exceeded 70% of the total starter cells (Additional file 3: Fig. S3a).The variability of the retrograde tracing results among different mice was detected by the total number of starter cells.The virus infection efficiency was confirmed by the ratio of total input neurons to the starter cells (Additional file 3: Fig. S3b).Relatively consistent results across different samples indicated a stable virus infection efficiency (Additional file 3: Fig. S3b).Moreover, the specificity of the virus strategy was validated by several control experiments, including the absence of RV (Additional file 3: Fig. S3c, d), the absence of Cre provided by mice (Additional file 3: Fig. S3e), or retrograde labeling targeting in adjacent subregion (Additional file 3: Fig. S3f ).These results confirmed that the monosynaptic labeling was specific for vGlut2 + neurons in the LHb and that our strategy displayed minimal nonspecific labeling.
We observed retrograde labeling signals in various brain subregions in the ipsilateral and contralateral hemispheres of both sexes, including the cortex, striatum, pallidum, thalamus, hypothalamus, midbrain, pons, medulla, and cerebellum (Fig. 1d, e; Additional file 5: Fig. S5a, c; Additional file 10: Table 1; Additional file 12: Table 3).These findings are consistent with the input circuits to the LHb neurons [27].We found ipsilateral inputs biased in the inputs of LHb vGlut2 neurons, contributing to almost 80% of total inputs, which were distributed mainly in 33 brain subregions throughout the brain (Fig. 1d, e).The inputs from the entopeduncular nucleus (EPN) and LHA contributed the highest proportion of the total input to LHb vGlut2 neurons (Fig. 1e).Consistently, the maximum inputs were mainly distributed from bregma − 0.5 to -1.5 mm, where the pallidum and hypothalamus were located (Fig. 1f ).We also found that no sex differences were presented in ipsilateral and contralateral inputs across classified groups (Fig. 1e).On the other hand, a small proportion of brain subregions bilaterally innervated the LHb vGlut2 neurons, such as the VP, diagonal band nucleus (DBN), lateral preoptic area (LPO), and LHA (Additional file 5: Fig. S5c).We next assessed the ipsilateral and contralateral input connectivity of the LHb vGlut2 neurons from 11 large brain regions to gain an overview of the overall connectivity of LHb vGlut2 neurons (Additional file 5. Fig. S5a).We found that LHb vGlut2 neurons were heavily connected with the pallidum, hypothalamus, and midbrain without showing sex differences.
The habenula region used to be separated into two distinct subregions, the MHb and the LHb.To compare the input pattern with LHb vGlut2 neurons, we explored the inputs of adjacent MHb vGlut2 neurons (Additional file 4: Fig. S4a, b).We found that MHb mainly received monosynaptic inputs from the medial septum (MS), DBN, triangular septal nucleus (TS), anterior group of the dorsal thalamus (ATN), interpeduncular nucleus (IPN), and posterodorsal tegmental nucleus (PDTg) (Additional file 4: Fig. S4c, d).Overall, the input map of MHb vGlut2 neurons was vastly distinct from the LHb vGlut2 neurons.

Whole brain mapping of the LHb vGlut2 output in male and female mice
To investigate the whole brain output of LHb vGlut2 neurons, we employed an anterograde tracing strategy by injecting the AAV virus expressing presynaptic marker synaptophysin conjugated to EGFP and membranebound tdTomato into the unilateral LHb of Vglut2-ires-Cre mice (Fig. 2a).The tdTomato signals were found in the soma (Fig. 2b; Additional file 6: Fig. S6; Additional file 7: Fig. S7) and the downstream axons, while the presynaptic boutons originating from the starter cells were labeled by the EGFP signals (Fig. 2d).The AAV starter cells were predominantly distributed in the central to posterior part of the LHb in both sexes (Fig. 2c).
On a whole brain scale, we found that LHb vGlut2 neurons projected to over 30 downstream regions, spanning over seven brain regions and fiber tracts in male and female mice (Fig. 2d; Additional file 11: Table 2).These extensive projections of LHb vGlut2 neurons, ranging from the anterior cortex to the caudal pons, targeted voluminous regions of the midbrain and pons area (Additional file 11: Table 2; Additional file 12: Table 3).Moreover, the highest density of outputs consistently arose from bregma − 3.0 to -4.5 mm, where the midbrain and pons are located (Fig. 2f; Additional file 5. Fig. S5b).Regarding the predominant output subregions, the efferent projections were enriched in a specific subset, including the mediodorsal nucleus of the thalamus (MD), VTA, caudal rostral-medial tegmental (cRMTg), and median raphe nucleus (P/MnR) (Fig. 2e).Particularly, the normalized output density of the ipsilateral MD, VTA, cRMTg, and P/MnR, comprising approximately 10%, 10%, 25%, and 10% of the total outputs, respectively (Fig. 2e).Overall, midbrain and pons contributed to the highest projection density, accounting for approximately 70% of the total outputs.
Comparing with the input map of LHb vGlut2 neurons, we found there was an absence of efferent to the cerebellum, but a presence of presynaptic boutons from the LHb vGlut2 neurons in fiber tracts (Additional file 5. Fig. S5a, b).Similarly, we found no sex differences in output density across all brain subregions, hence the input and output strength of LHb vGlut2 neurons between males and females seem comparable.

Axonal bouton dynamics of LHb vGlut2 neurons in the whole brain of both sexes
The execution of neuronal function depends on synaptic communication within the cell population [31,32].The glutamatergic circuitry of corticocortical and trans-thalamic pathways plays a crucial role in information transmission and processing networks in the brain, falling into two main categories [33].One category conveys information through large synaptic boutons, while the other utilizes small terminal boutons to modulate cortical connections.With distinct features of boutons (Fig. 3a), we classified brain subregions into different clusters according to various features of LHb vGlut2 presynaptic boutons, including bouton size (Additional file 8. Fig. S8a), number (Additional file 8. Fig. S8b), and occupied area (Additional file 8. Fig. S8c), respectively, as well as combination of these three features (Fig. 3a, b).
Regarding bouton size, we identified four distinct clusters (Additional file 8. Fig. S8a).Specifically, the cRMTg displayed the largest bouton size among all downstream subregions, while the MD, P/MnR, CLi, and fiber tracts showed relatively large bouton sizes.Conversely, areas such as the mPFC, VP, lateral geniculate nucleus (GENv), and the other nine nuclei showed small bouton size (Fig. 3a, d; Additional file 8. Fig. S8a).The unique morphology of presynaptic boutons indicates that projections from LHb vGlut2 neurons may possess diverse functional properties.In addition to bouton size, the efficacy of synaptic functions is also influenced by the number of synapses [34].Remarkably, we observed a high presence The details of abbreviations for brain subregions can be seen in the list of abbreviations of presynaptic boutons in the caudoputamen (CPU) (Fig. 3a; Additional file 8. Fig. S8a).Moreover, most brain subregions showed small occupied areas, indicating specific spatial characteristics of these efferents from LHb (Fig. 3a; Additional file 8. Fig. S8a).
Combined with these three boutons features collectively, we identified five distinct clusters for these brainwide subregions (Fig. 3b).Cluster I displayed a large bouton size and relatively moderate distribution, notably in the MD and VTA (Fig. 3c, d).Cluster II showed a relatively small bouton size and restricted spatial location, particularly in the CPU (Fig. 3b, c), indicating potential spatial specificity in the function of this connection.Cluster III displayed a moderate bouton size and broad spatial distribution, such as the P/MnR (Fig. 3b, c, d).Cluster IV demonstrated moderate to dense numbers but tiny boutons, involving regions like the dorsal nucleus raphe (DRN), zona incerta (ZI), LPO, and LHA (Fig. 3b,  c, d).Cluster V demonstrated a dense and widespread distribution with a large bouton size, among which the cRMTg is a typically downstream nucleus (Fig. 3b, c, d).These distinct clusters with varying projecting patterns suggested that LHb functions through unique innervating characteristics or different subsets of the neuronal population.

Whole-brain connectome of LHb vGlut2 neurons
To establish a refined connective map of LHb vGlut2 neurons, we delineated the whole-brain spatial distribution of ipsilateral inputs and outputs of LHb vGlut2 neurons in male and female mice (Fig. 4a).Based on the calculated proportion of inputs and outputs in the resided anatomy group, we constructed a whole-brain connection of LHb vGlut2 neurons (Fig. 4b, c).Regarding inputs to and outputs from LHb vGlut2 neurons, we found that males and females show high similarity with a strong correlation (Fig. 5a).We then investigated the reciprocal connection of the LHb vGlut2 neurons by correlating inputs with outputs in male and female mice.However, we found that the majority of brain subregions were often not bidirectionally connected with LHb vGlut2 neurons in both sexes (Fig. 5b).Overall, the connective subregions of LHb vGlut2 neurons can be classified into three categories: I received reciprocal connection, II only projected to the LHb, and III only received synaptic innervation from the LHb.According to these categories, we then compared input and output patterns in both sexes.

Comparison of the input and output connection pattern of LHb vGlut2 neurons
To explore the similarities and dissimilarities in the connectivity of LHb vGlut2 neurons between males and females, we performed a hierarchical cluster analysis of the correlation coefficients.We examined 16 brain subregions with consistent infection efficiency in various samples from both groups for pairwise comparisons (Fig. 5c-f ).The cluster data did not show complete consistency in either the inputs or outputs between males and females.The inputs of LHb vGlut2 neurons formed obvious but inconsistent clusters in both sexes, suggesting the presence of unique collateral connection patterns (Fig. 5c, d).Furthermore, GENv and mPFC formed separate clusters in both sexes, indicating specific functions associated with these different clusters (Fig. 5c, d).In terms of outputs, GENv and ZI clustered together in both males and females (Fig. 5e, f ).Additionally, there were no apparent sex differences in the input and output patterns across the whole brain for male and female mice (Additional file 9. Fig. S9a, b).

Discussion
In this study, we used monosynaptic retrograde tracing and anterograde tracing to analyze the whole-brain connectivity map of LHb vGlut2 neurons in both sexes.We found that the input and output connections of LHb vGlut2 neurons are highly similar between males and females.In particular, we identified projections from the LHb vGlut2 neurons to the MD, which were neglected previously.Furthermore, we clustered the brain-wide output nuclei into five clusters based on specific presynaptic bouton features, including bouton size, number, and occupied area.Differences in connectivity patterns are related to specific pathways of information processes underlying specific behaviors and functions.Our work lays the groundwork for exploring the relationships between cell heterogeneity of the LHb and anatomical connectivity in specific behavior.
Our study revealed that the LHb vGlut2 neurons primarily received inputs from the forebrain [16] and projected to the midbrain [7,[35][36][37], which is consistent with previous studies.The densest inputs of LHb vGlut2 neurons were LHA and EPN, which are closely related to the affective process [38][39][40].However, the densest outputs from LHb vGlut2 neurons were in the VTA, cRMTg, and P/MnR, which are highly related to the reward process [3,7,41,42].Furthermore, we found that there were few reciprocal connections of LHb vGlut2 neurons, indicating a weak feedback circuit of LHb vGlut2 neurons.
Although a previous study reported sexual dimorphism in inputs to the LHb [27], our findings did not reveal significant sex differences in whole-brain inputs to the LHb vGlut2 neurons.Interestingly, both males and females exhibited highly similar connective features in terms of upstream and downstream connections in both ipsilateral and contralateral areas.These inconsistencies could be attributed to variations in cell types, injection sites, and injection volumes used in different studies.The differences in injection sites and targeted subpopulations between the two studies led us to speculate that specific subsets of GABAergic neurons or other subpopulations in the LHb might influence the observed sex-specific input patterns.Although this study did not reveal sex differences in connections between the LHb and midbrain, a previous study highlighted sex differences in LHbinduced inhibition of the midbrain dopamine neurons firing in rats, which is reduced in females compared to males [43].These suggested that sex-specific responses by the LHb electrical stimuli may be influenced by factors such as sex hormones, cell identification, and transcript profiling.Clinically relevant sex differences have been noted in the prevalence or severity of several of the conditions including depression [44], schizophrenia [45], and addiction [46].Understanding the functionality associated with LHb circuits has broad applications in the field of neurological and mental health.
We also discovered that the connectivity map of LHb vGlut2 neurons formed distinct clusters and displayed area-specificity in both sexes.For example, the LHb vGlut2 projecting neurons from LHA were mainly distributed in anterior parts but dispersed in nearly all areas of the LHA.However, the LHA presynaptic output boutons from the LHb vGlut2 neurons targeted nearly all areas but with relatively sparse density.Similarly, the output boutons to the LPO, ZI, P/MnR, and cRMTg displayed a spatial distribution pattern that covered almost the entire area, which could provide valuable insights into their functions.Moreover, we found that one target of the presynaptic boutons originating from the LHb vGlut2 neurons was the anterior lateral part of the MD.Previous studies have indicated that MD is implicated in associative memory encoding and retrieval [47], as well as the transmission of sensory information related to sensations like pain and itch [48], which suggested that the LHb-MD pathway might play a role in regulating memory processing [49,50].
The characteristics of boutons were thought to be related to electrophysiological functions and synaptic strength in cortical-cortical and cortical-thalamus connection [33,51,52].We observed that few output brain subregions received relatively large presynaptic boutons from the LHb vGlut2 neurons, while most output nuclei displayed small presynaptic bouton features.Our findings revealed that the LHb vGlut2 -VTA, P/MnR, and cRMTg circuits accounted for a relatively large part of total output density with large boutons.Previous studies have demonstrated that the LHb sends direct glutamatergic projections to both GABAergic and dopaminergic neurons in the VTA [9,53,54], although they are much sparser than the LHb's projection to cRMTg.These circuits are involved in carrying information related to aversive stimuli [55], passive and conditioned behavioral avoidance induced by specific paradigm [29,56,57].In addition, we found that the presynaptic boutons of LHb vGlut2 -DRN, Zl, LPO, and LHA displayed dense numbers but small size and wide volume, indicating that these glutamatergic pathways may play a critical role from LHb vGlut2 neurons.Furthermore, the size of synaptic boutons is associated with the diameter of axons [58], which is crucial in determining conduction velocity [59].It is implied that LHb circuits with larger boutons have the potential to transmit signals more rapidly.
The investigation presented here meticulously compared the connective pattern in both sexes, however, several limitations must be considered.Firstly, the inability to quantify the proportion of each subregion output and reconstruct it due to the lack of volume calculation of the whole-brain output.It is necessary to use more precise imaging methods to create a more comprehensive connection reconstruction of LHb vGlut2 neurons.Additionally, glutamatergic cells within the LHb expressed the mRNA of either vGlut2 or vGlut3 [60] with heterogeneous functions in response to different stimuli.Our study did not delineate the connectivity map of LHb vGlut3 neurons.Furthermore, while we excluded the specific input of the MHb from our statistical results, it is inevitable that some input neurons from both the LHb and MHb were excluded, albeit in small numbers.Moreover, the output strength of weak connection from the LHb may be influenced by common projections from the pretectal regions.It is also important to note that the potential for coincidental association may introduce false positive correlations, and observable correlations may not imply a causal relationship between variables.Therefore, further investigation is essential for experimental validation and interpretation.

Perspective and insight
This study depicted the whole-brain inputs and outputs, along with the spatial distribution and connective pattern of the LHb vGlut2 neurons in both sexes.Furthermore, previously overlooked circuit connections were identified, which could be significant in the physiological    Outputs vs. outputs in males (e) or females (f ).The details of abbreviations for brain subregions can be seen in the list of abbreviations functions and pathologies associated with LHb vGlut2 neurons.In summary, our anatomical description reinforces the functional role of the LHb as a key hub in complex behaviors and mental disorders.The consistent connections observed in both male and female mice imply that the fundamental organizational principle of the neural network of vGlut2 + neurons in the LHb is conserved.

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See figure on previous page.)Fig. 1 Whole-brain monosynaptic ipsilateral and contralateral inputs to the LHb vGlut2 neurons in both sexes.(a) Schematic of monosynaptic rabies virus tracing strategy.(b) Representative fluorescent micrographs of coronal brain slice containing injection site in the LHb (left) and clear morphology of starter cell soma (right).Scale bar (left) = 200 μm, scale bar (right) = 50 μm.(c) Heatmap showing starter cell distribution in each coronal section including LHb of each sample.Each row is from one sample.(d) Representative examples of brain-wide ipsilateral (Ipsi) monosynaptic inputs to LHb vGlut2 neurons in one mouse.Scale bars, 200 μm.(e) Quantification of ipsilateral and contralateral LHb vGlut2 projecting neurons expressed as a percentage of the total input cells in the whole brains of both male and female mice.(f) Input proportion (top) and area under the curve (AUC) (bottom) plots along the anterior-posterior axis covering the entire brain subregions that project to the LHb vGlut2 neurons.Data are shown as mean ± s.e.m., n = 6 in each group.

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See figure on previous page.)Fig. 2 Whole-brain outputs of LHb vGlut2 neurons in both sexes.(a) Schematic of anterograde tracing strategy labeling outputs of LHb vGlut2 neurons.(b) Representative image showing the virus injection site in the LHb (left) and clear morphology of starter cell soma (right).Scale bar (left) = 200 μm, scale bar (right) = 50 μm.(c) Heatmap showing anterograde-tracing-virus infected cell distribution in each coronal section including LHb of each sample.Each row is from one sample.n = 6 in each group.(d) Representative examples of brain-wide ipsilateral outputs from LHb vGlut2 neurons in one mouse.Scale bars, 200 μm.(e) Quantification of the normalized density of ipsilateral and contralateral downstream brain subregions from LHb vGlut2 neurons in the whole brains of both sexes.(f) Normalized density of LHb vGlut2 outputs (top) and area under the curve (AUC) (bottom) from the anterior-posterior axis covering the entire efferent brain regions.The details of abbreviations for brain subregions can be seen in the list of abbreviations

Fig. 3
Fig. 3 Presynaptic boutons features from LHb vGlut2 neurons in the whole brain.(a) Quantification of normalized bouton size (left), number (middle), and occupied area (right) in each of the 30 identified target subregions.n = 6 in each group.(b) Cluster of innervating characteristics of the downstream nucleus including bouton size, number, and occupied area.(c) Representative images showing the spatial distribution of presynaptic boutons in subregions from different clusters.Scale bars, 200 μm.(d) Representative images showing different presynaptic boutons size from brain subregions of different clusters.Scale bars, 50 μm.The details of abbreviations for brain subregions can be seen in the list of abbreviations

Fig. 4
Fig. 4 Whole brain LHb vGlut2 neurons connectivity map.(a) Coronal sections depicting the spatial distribution of ipsilateral inputs and outputs in both sexes.Left, input; right, output; blue dot, male; orange dot, female.(b) Sankey network showing the connective strength of subregions (the width of the curve represents connective strength).(c) Schematic showing connectivity map of LHb vGlut2 neurons.The details of abbreviations for brain regions can be seen in the list of abbreviations

Fig. 5
Fig. 5 Whole-brain connection pattern of LHb vGlut2 connectivity.(a) Comparison of inputs (left) and outputs (right) between females and males.(b) Comparison of inputs and outputs in male (left) and female (right).(c, d) Matrices of hierarchically clustered pair-wise correlation coefficients (Spearman) of inputs.Inputs vs. inputs in males (c) or females (d).(e, f) Matrices of hierarchically clustered pair-wise correlation coefficients (Spearman) of outputs.Outputs vs. outputs in males (e) or females (f ).The details of abbreviations for brain subregions can be seen in the list of abbreviations